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14D2I32 - Behaviour and molecular ecology of bees

Course specification
Course title Behaviour and molecular ecology of bees
Acronym 14D2I32
Study programme DOCTORAL ACADEMIC STUDIES
Module
Type of study PhD academic studies
Lecturer (for classes)
Lecturer/Associate (for practice)
    Lecturer/Associate (for OTC)
      ESPB 4.0 Status elective
      Condition Enrolled semester in which the subject is attended Oblik uslovljenosti
      The goal The subject aims to provide students the knowledge about application of molecular genetics methods in exploration of ecology, behaviour and protection of honey bees.
      The outcome Student should become capable for application of molecular genetic methods in analyses of behaviour and ecology of bees, as well as in exploration of bee pathogens aimed to maintain health honey bee colonies.
      Contents
      Contents of lectures Theoretical instructions: Genetic analyses of honey bees. Mitochondrial and nuclear DNA – evaluation of genetic diversity and protection of honey bee ecotypes. Gene flow as an indicator of migratory beekeeping, commercial breeding and queen introduction. Molecular genetics in bee breeding and selection programs aimed to increase disease resistance. Behavioural genetics of bees. Heritability coefficient of hygienic and grooming behaviours in suppression of honey bee mite reproduction. Molecular characterization and typization of pathogens of bees and bee brood.
      Contents of exercises Independent research activities: DNA isolation, amplification of conserved regions of mitochondrial DNA, amplification of nuclear DNA microsatellites (PCR, real-time PCR, RT-PCR, PCR-RFLP, LAMP), visualisation of results by gel electrophoresis and DNA sequencing, analysis of the obtained results with an aim to analyse genome and molecular diversity of bees. Molecular detection and identification of bee pathogens and parasites.
      Literature
      1. Stanimirović Z, Soldatović B, Vučinić M: Bee biology-Honey bee, Medicinska knjiga-Medicinske komunikacije, Beograd, 2000.
      2. Baker A (ed): Molecular Methods in Ecology, Blackwell Science, 2000. (Original title)
      3. Hoy MA: Insect Molecular Genetics: An Introduction to Principles and Applications, 2nd ed. Academic Press/Elsevier, 2003. (Original title)
      4. Behura SK: Molecular marker systems in insects: current trends and future avenues. Molecular Ecology 15 (11) 3087–3113, 2006. (Original title)
      Number of hours per week during the semester/trimester/year
      Lectures Exercises OTC Study and Research Other classes
      2
      Methods of teaching Theoretical instruction through preparation of seminary elaborate, public interpretation and critical discussion. Independent research activities: performing experiment, analysis and discussion of the results.
      Knowledge score (maximum points 100)
      Pre obligations Points Final exam Points
      Activites during lectures 10 Test paper 70
      Practical lessons Oral examination
      Projects
      Colloquia
      Seminars 20