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14D2I31 - Molecular markers in analysis and detection of racial affiliation and pedigree

Course specification
Course title Molecular markers in analysis and detection of racial affiliation and pedigree
Acronym 14D2I31
Study programme DOCTORAL ACADEMIC STUDIES
Module
Type of study PhD academic studies
Lecturer (for classes)
Lecturer/Associate (for practice)
    Lecturer/Associate (for OTC)
      ESPB 4.0 Status elective
      Condition Enrolled semester in which the subject is attended Oblik uslovljenosti
      The goal The subject aims to provide students the knowledge about application of molecular genetics techniques in parentage testing, kinship analysis and affirming of breed affiliation.
      The outcome Student should understand the role of parentage testing in animal pedigree verification and breeding programs. Finnaly, student have to be acquainted with the factors that affect the accuracy of the test for parentage determining and pedigree analysis. Student should become capable to perform parentage testing, kinship analysis and affirming of breed affiliation.
      Contents
      Contents of lectures Theoretical instructions: From Mendel to molecular markers. Types of molecular markers. Nuclear and mitochondrial DNA and correlation analyses between genetic polymorphisms and specific phenotypic characteristics. Application of data obtained by analyses of DNA polymorphisms in determination of kinship, parentage and pedigree.
      Contents of exercises Independent research activities: DNA isolation, amplification of conserved regions of mitochondrial DNA, amplification of nuclear DNA microsatellites (PCR, real-time PCR, RT-PCR, PCR-RFLP, LAMP), visualisation of results by gel electrophoresis and DNA sequencing, analysis of the obtained results with aimed to kinship, parentage and pedigree analyses.
      Literature
      1. Đelić N, Stanimirović Z: Principi genetike, Fakultet veterinarske medicine, Univerzitet u Beogradu, Elit-Medica, Beograd, 2004. (Original title)
      2. Wilson K, Walker J: Principles and Techniques of Biochemistry and Molecular Biology, 7h edition, Cambridge University Press, 2010. (Original title)
      3. Watson JD, Baker TA, Bell SP, Gann A, Levine M, Losick R: Molecular Biology of the Gene, 7th Edition, Pearson Education, 2013. (Original title)
      4. Farkas DH, DNA from A to Z, Amer Assoc for Clinical Chemistry, 2004. (Original title)
      5. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P: Molecular Biology of the Cell, 5th ed., Garland Science, New York, 2007. (Original title)
      Number of hours per week during the semester/trimester/year
      Lectures Exercises OTC Study and Research Other classes
      2
      Methods of teaching Theoretical instruction through preparation of seminary elaborate, public interpretation and critical discussion. Independent research activities: performing experiment, analysis and discussion of the results.
      Knowledge score (maximum points 100)
      Pre obligations Points Final exam Points
      Activites during lectures 10 Test paper 70
      Practical lessons Oral examination
      Projects
      Colloquia
      Seminars 20

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